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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCNMB4 Monoclonal antibody

KCNMB4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4H7G3

Cat no : 60122-1-Ig

Synonyms

BK channel subunit beta 4, BKbeta4, Hbeta4, K(VCA)beta 4, KCNMB4, Maxi K channel subunit beta 4, Slo beta 4

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using KCNMB4 Monoclonal antibody (60122-1-Ig)

    WB analysis of fetal human brain using 60122-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using KCNMB4 Monoclonal antibody (60122-1-Ig)

    WB analysis of human brain using 60122-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using KCNMB4 Monoclonal antibody (60122-1-Ig)

    IHC staining of human brain using 60122-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KCNMB4 Monoclonal antibody (60122-1-Ig)

    IHC staining of human brain using 60122-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNMB4 Monoclonal antibody (60122-1-Ig)

    IHC staining of mouse brain using 60122-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNMB4 Monoclonal antibody (60122-1-Ig)

    IHC staining of mouse brain using 60122-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

60122-1-Ig targets KCNMB4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KCNMB4 fusion protein Ag5618 相同性解析による交差性が予測される生物種
Full Name potassium large conductance calcium-activated channel, subfamily M, beta member 4
Calculated molecular weight 24 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC042446
Gene symbol KCNMB4
Gene ID (NCBI) 27345
RRIDAB_2280832
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KCNMB4 antibody 60122-1-IgDownload protocol
IHC protocol for KCNMB4 antibody 60122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Cytoprotective effects of the flavonoid quercetin by activating mitochondrial BKCa channels in endothelial cells.

Authors - Rafał Paweł Kampa
View Article
humanWB

Mitochondrion

Deficiency of the BKCa potassium channel displayed significant implications for the physiology of the human bronchial epithelium

Authors - Kamila Maliszewska-Olejniczak
View Article