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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNQ2 Monoclonal antibody (66774-1-Ig)

    WB analysis using 66774-1-Ig (same clone as 66774-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66774-1-Ig (KCNQ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNQ2 Monoclonal antibody (66774-1-Ig)

    IHC staining of mouse brain using 66774-1-Ig (same clone as 66774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66774-1-Ig (KCNQ2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNQ2 Monoclonal antibody (66774-1-Ig)

    IHC staining of mouse brain using 66774-1-Ig (same clone as 66774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66774-1-Ig (KCNQ2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KCNQ2 Monoclonal antibody (66774-1-Ig)

    IF Staining of U2OS using 66774-1-Ig (same clone as 66774-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using KCNQ2 antibody (66774-1-Ig, Clone: 1D4A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50721-2

    Cytometric bead array standard curve of MP50721-2

    Cytometric bead array standard curve of MP50721-2, KCNQ2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66774-4-PBS. Detection antibody: 66774-1-PBS. Standard:Ag25781. Range: 0.781-100 ng/mL.

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KCNQ2 Monoclonal antibody, PBS Only (Detector)

KCNQ2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66774-1-PBS
Clone No.1D4A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

HNSPC, ENB1, EBN1, EBN, BFNC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66774-1-PBS targets KCNQ2 as part of a matched antibody pair:

MP50721-2: 66774-4-PBS capture and 66774-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KCNQ2 fusion protein Ag25781 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, KQT-like subfamily, member 2
Calculated molecular weight 96 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC000699
Gene Symbol KCNQ2
Gene ID (NCBI) 3785
RRIDAB_2882120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43526
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.