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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using KCTD10 Polyclonal antibody (27279-1-AP)

    WB analysis of Jurkat using 27279-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using KCTD10 Polyclonal antibody (27279-1-AP)

    WB analysis of U2OS using 27279-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KCTD10 Polyclonal antibody (27279-1-AP)

    WB analysis of MCF-7 using 27279-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using KCTD10 Polyclonal antibody (27279-1-AP)

    WB analysis of Jurkat using 27279-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KCTD10 Polyclonal antibody (27279-1-AP)

    WB analysis of HEK-293 using 27279-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using KCTD10 Polyclonal antibody (27279-1-AP)

    WB analysis of K-562 using 27279-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KCTD10 Polyclonal antibody (27279-1-AP)

    WB analysis of HeLa using 27279-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KCTD10 Polyclonal antibody (27279-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27279-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KCTD10 Polyclonal antibody (27279-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27279-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

KCTD10 Polyclonal antibody

KCTD10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27279-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Synonyms

ULR061, Potassium channel tetramerization domain-containing protein 10, MSTP028, hBACURD3, BTB/POZ domain-containing protein KCTD10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, U2OS cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27279-1-AP targets KCTD10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCTD10 fusion protein Ag25971 相同性解析による交差性が予測される生物種
Full Name potassium channel tetramerisation domain containing 10
Calculated molecular weight 313 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC040062
Gene Symbol KCTD10
Gene ID (NCBI) 83892
RRIDAB_2880827
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3F6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis.

Protocols

Product Specific Protocols
WB protocol for KCTD10 antibody 27279-1-APDownload protocol
IHC protocol for KCTD10 antibody 27279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Unbiased interrogation of functional lysine residues in human proteome

Authors - Ying Bao
View Article
humanWB

Nat Commun

Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.

Authors - Xin Wu
  • KO Validated
View Article
mouseWB

Front Cell Dev Biol

Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.

Authors - Yue Liu
  • KD Validated
View Article
human,ratWB

Genet Res (Camb)

Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis

Authors - Yun Feng
  • KD Validated
View Article