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KD/KO Validated

KCTD12 Polyclonal antibody

Name: KCTD12 Polyclonal antibody
Brand: Proteintech
SKU: 15523-1-AP

KCTD12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15523-1-AP

Synonyms

Pfetin, PFET1, C13orf2, BTB/POZ domain-containing protein KCTD12

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of mouse brain using 15523-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15523-1-AP
Hela cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15523-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15523-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15523-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15523-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15523-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human bowen disease using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15523-1-AP
Immunofluorescent analysis of Hela cells, using KCTD12 antibody 15523-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 15523-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, MCF-7 cells, human brain tissue, HEK-293 cells, Hela cells, rat brain tissue
Positive IHC detected in	human bowen disease, human intrahepatic cholangiocarcinoma tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IHC	See 2 publications below
IF	See 5 publications below

Product Information

15523-1-AP targets KCTD12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KCTD12 fusion protein Ag5716 相同性解析による交差性が予測される生物種
Full Name	potassium channel tetramerisation domain containing 12
Calculated molecular weight	36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC013764
Gene symbol	KCTD12
Gene ID (NCBI)	115207
RRID	AB_2132006
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for KCTD12 antibody 15523-1-AP	Download protocol
IHC protocol for KCTD12 antibody 15523-1-AP	Download protocol
IF protocol for KCTD12 antibody 15523-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF	Oncogene KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition. Authors - Y Zhong KD Validated View Article
mouse	WB,IF	Pharmacol Res The effects of Kctd12, an auxiliary subunit of GABAB receptor in dentate gyrus on behavioral response to chronic social defeat stress in mice. Authors - Si-Long Deng View Article
mouse	WB,IF	J Neurosci KCTD8 and KCTD12 Facilitate Axonal Expression of GABAB Receptors in Habenula Cholinergic Neurons. Authors - Yuqi Ren KO Validated View Article
human	WB,IHC	Sci Rep KCTD12 Regulates Colorectal Cancer Cell Stemness through the ERK Pathway. Authors - Liping Li KD Validated View Article
mouse	IF	Front Mol Neurosci The Electrophysiological Determinants of Corticospinal Motor Neuron Vulnerability in ALS. Authors - Javier H Jara View Article
mouse	WB	PLoS One Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus. Authors - Kelly A Hamilton View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCTD12 Polyclonal antibody

KCTD12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 15523-1-AP

WB analysis of HeLa using 15523-1-AP

WB analysis of human brain using 15523-1-AP

WB analysis of HEK-293 using 15523-1-AP

WB analysis of mouse brain using 15523-1-AP

WB analysis of MCF-7 using 15523-1-AP

WB analysis of rat brain using 15523-1-AP

IHC staining of human bowen disease using 15523-1-AP

IHC staining of human bowen disease using 15523-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP

IHC staining of human stomach using 15523-1-AP

IHC staining of human stomach using 15523-1-AP

IF Staining of HeLa using 15523-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.

The effects of Kctd12, an auxiliary subunit of GABAB receptor in dentate gyrus on behavioral response to chronic social defeat stress in mice.

KCTD8 and KCTD12 Facilitate Axonal Expression of GABAB Receptors in Habenula Cholinergic Neurons.

KCTD12 Regulates Colorectal Cancer Cell Stemness through the ERK Pathway.

The Electrophysiological Determinants of Corticospinal Motor Neuron Vulnerability in ALS.

Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus.