• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCTD14 Polyclonal antibody

KCTD14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21111-1-AP

Synonyms

BTB/POZ domain-containing protein KCTD14

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    WB analysis of mouse skeletal muscle using 21111-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    WB analysis of mouse brain using 21111-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    WB analysis of mouse small intestine using 21111-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    IHC staining of human stomach cancer using 21111-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    IHC staining of human stomach cancer using 21111-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    IHC staining of human ovary cancer using 21111-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    IHC staining of human ovary cancer using 21111-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 21111-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KCTD14 Polyclonal antibody (21111-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 21111-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse brain tissue, mouse small intestine tissue
Positive IHC detected inhuman stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21111-1-AP targets KCTD14 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCTD14 fusion protein Ag14265 相同性解析による交差性が予測される生物種
Full Name potassium channel tetramerisation domain containing 14
Calculated molecular weight 225 aa, 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC001929
Gene symbol KCTD14
Gene ID (NCBI) 65987
RRIDAB_10733343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KCTD14, or potassium channel tetramerization domain containing 14, is a member of the KCTD family of proteins. This family consists of 25 members in humans, many of which are only partially characterized . KCTD14, like other members of the KCTD family, contains a conserved domain known as the BTB (Broad complex, Tramtrak, and Bric-a-brac)/POZ (poxvirus zinc finger) domain, which is crucial for protein oligomerization and establishing protein-protein interactions. KCTD14 has been implicated in various biological functions, and recent research suggests a potential role in cancer. Although not as extensively studied as some other KCTD members, KCTD14 has been noted in database analyses to potentially play a protumor role in ovarian cancer. Specifically, the COSMIC database reports a copy number variation (CNV) gain in 4.5% of ovarian cancers, and the GENT2 database indicates a fold change (FC) of 1.5 in expression, with a p-value less than 0.001, suggesting a possible association with ovarian cancer.

Protocols

Product Specific Protocols
WB protocol for KCTD14 antibody 21111-1-APDownload protocol
IHC protocol for KCTD14 antibody 21111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols