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  • Featured Product
  • KD/KO Validated

KCTD15 Polyclonal antibody

KCTD15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20128-1-AP

Synonyms

KCTD15

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KCTD15 Polyclonal antibody (20128-1-AP)

    WB analysis of HEK-293 using 20128-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using KCTD15 Polyclonal antibody (20128-1-AP)

    WB analysis of mouse lung using 20128-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using KCTD15 Polyclonal antibody (20128-1-AP)

    IHC staining of mouse spleen using 20128-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20128-1-AP (KCTD15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using KCTD15 Polyclonal antibody (20128-1-AP)

    IHC staining of mouse spleen using 20128-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20128-1-AP (KCTD15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KCTD15 Polyclonal antibody (20128-1-AP)

    IF Staining of HepG2 using 20128-1-AP

    Immunofluorescent analysis of HepG2 cells, using KCTD15 antibody 20128-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse lung tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20128-1-AP is KCTD15 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCTD15 fusion protein Ag13962 相同性解析による交差性が予測される生物種
Full Name potassium channel tetramerisation domain containing 15
Calculated molecular weight 283 aa, 32 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC001185
Gene symbol KCTD15
Gene ID (NCBI) 79047
RRIDAB_10646430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KCTD15 antibody 20128-1-APDownload protocol
IHC protocol for KCTD15 antibody 20128-1-APDownload protocol
IF protocol for KCTD15 antibody 20128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogenesis

KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2.

Authors - Eleonora Spiombi
  • KD Validated
View Article