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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KDELC2 Polyclonal antibody (23345-1-AP)

    WB analysis of HeLa using 23345-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KDELC2 Polyclonal antibody (23345-1-AP)

    WB analysis of HeLa using 23345-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using KDELC2 Polyclonal antibody (23345-1-AP)

    WB analysis of human placenta using 23345-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using KDELC2 Polyclonal antibody (23345-1-AP)

    WB analysis of SMMC-7721 using 23345-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KDELC2 Polyclonal antibody (23345-1-AP)

    IHC staining of human breast cancer using 23345-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using KDELC2 Polyclonal antibody (23345-1-AP)

    IHC staining of human placenta using 23345-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using KDELC2 Polyclonal antibody (23345-1-AP)

    IHC staining of human placenta using 23345-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KDELC2 Polyclonal antibody (23345-1-AP)

    IHC staining of human breast cancer using 23345-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KDELC2 Polyclonal antibody (23345-1-AP)

    IF Staining of HepG2 using 23345-1-AP

    Immunofluorescent analysis of HepG2 cells using 23345-1-AP (KDELC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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KDELC2 Polyclonal antibody

KDELC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23345-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein O-xylosyltransferase POGLUT3, Protein O-glucosyltransferase 3, POGLUT3, KDEL motif-containing protein 2, EC:2.4.2.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, SMMC-7721 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23345-1-AP targets KDELC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KDELC2 fusion protein Ag19929 相同性解析による交差性が予測される生物種
Full Name KDEL (Lys-Asp-Glu-Leu) containing 2
Calculated molecular weight 507 aa, 59 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC036526
Gene Symbol KDELC2
Gene ID (NCBI) 143888
RRIDAB_2879260
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4H8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KDELC2 antibody 23345-1-APDownload protocol
IHC protocol for KDELC2 antibody 23345-1-APDownload protocol
IF protocol for KDELC2 antibody 23345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols