KDM1 Monoclonal antibody
KDM1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2F10A12
Cat no : 67037-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, HepG2 cells, PC-12 cells, 4T1 cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
67037-1-Ig targets KDM1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KDM1 fusion protein Ag14778 相同性解析による交差性が予測される生物種 |
| Full Name | lysine (K)-specific demethylase 1 |
| Calculated molecular weight | 876 aa, 95 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC040194 |
| Gene symbol | KDM1 |
| Gene ID (NCBI) | 23028 |
| RRID | AB_2882351 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM1 antibody 67037-1-Ig | Download protocol |
| IHC protocol for KDM1 antibody 67037-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci MicroRNA-325 facilitates atherosclerosis progression by mediating the SREBF1/LXR axis via KDM1A. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hua (Verified Customer) (10-21-2020) | Observed band at the right size with a clean background.
 |
