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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KDM1 Monoclonal antibody (67037-1-Ig)

    WB analysis using 67037-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KDM1 Monoclonal antibody (67037-1-Ig)

    WB analysis using 67037-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KDM1 Monoclonal antibody (67037-1-Ig)

    IHC staining of human colon cancer using 67037-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KDM1 Monoclonal antibody (67037-1-Ig)

    IHC staining of human colon cancer using 67037-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KDM1 Monoclonal antibody (67037-1-Ig)

    IHC staining of human lung cancer using 67037-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using KDM1 Monoclonal antibody (67037-1-Ig)

    IHC staining of human prostate cancer using 67037-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KDM1 Monoclonal antibody (67037-1-Ig)

    IHC staining of human lung cancer using 67037-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KDM1 Monoclonal antibody (67037-1-Ig)

    IHC staining of human colon cancer using 67037-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KDM1 Monoclonal antibody (67037-1-Ig)

    IHC staining of human liver cancer using 67037-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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KDM1 Monoclonal antibody

KDM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67037-1-Ig
Clone No.2F10A12
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

AOF2, BHC110, KDM1, KDM1A, KIAA0601, LSD1, lysine (K) demethylase 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, HepG2 cells, PC-12 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, human liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67037-1-Ig targets KDM1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KDM1 fusion protein Ag14778 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 1
Calculated molecular weight 876 aa, 95 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC040194
Gene Symbol KDM1
Gene ID (NCBI) 23028
RRIDAB_2882351
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60341
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.

Protocols

Product Specific Protocols
WB protocol for KDM1 antibody 67037-1-IgDownload protocol
IHC protocol for KDM1 antibody 67037-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci

MicroRNA-325 facilitates atherosclerosis progression by mediating the SREBF1/LXR axis via KDM1A.

Authors - Yanhua Pu
View Article