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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KDM1B Monoclonal antibody

KDM1B Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E1A3

Cat no : 67886-1-Ig

Synonyms

AOF1, LSD2, C6orf193, 1E1A3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KDM1B Monoclonal antibody (67886-1-Ig)

    WB analysis using 67886-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using KDM1B Monoclonal antibody (67886-1-Ig)

    WB analysis of K-562 using 67886-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using KDM1B Monoclonal antibody (67886-1-Ig)

    WB analysis of C6 using 67886-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using KDM1B Monoclonal antibody (67886-1-Ig)

    WB analysis of NIH/3T3 using 67886-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SW 1990 cells using KDM1B Monoclonal antibody (67886-1-Ig)

    FC experiment of SW 1990 using 67886-1-Ig

    1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug Anti-HUMAN KDM1B (67886-1-Ig, Clone:1E1A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHCT 116 cells, K-562 cells, C6 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells
Positive FC (Intra) detected inSW 1990 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67886-1-Ig targets KDM1B in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen KDM1B fusion protein Ag30672 相同性解析による交差性が予測される生物種
Full Name amine oxidase (flavin containing) domain 1
Calculated molecular weight 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberNM_001364614
Gene symbol KDM1B
Gene ID (NCBI) 221656
RRIDAB_2918643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KDM1B antibody 67886-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols