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- KD/KO Validated
KDM3A/JMJD1A Polyclonal antibody
KDM3A/JMJD1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 12835-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 15 publications below |
| WB | See 35 publications below |
| IHC | See 11 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 6 publications below |
Product Information
12835-1-AP targets KDM3A/JMJD1A in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KDM3A/JMJD1A fusion protein Ag3581 相同性解析による交差性が予測される生物種 |
| Full Name | lysine (K)-specific demethylase 3A |
| Calculated molecular weight | 150 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC038297 |
| Gene symbol | KDM3A/JMJD1A |
| Gene ID (NCBI) | 55818 |
| RRID | AB_1072935 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC. (PMID:20823141) This antibody recognize the N-term of KDM3A(JMJD1A).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM3A/JMJD1A antibody 12835-1-AP | Download protocol |
| IHC protocol for KDM3A/JMJD1A antibody 12835-1-AP | Download protocol |
| IF protocol for KDM3A/JMJD1A antibody 12835-1-AP | Download protocol |
| IP protocol for KDM3A/JMJD1A antibody 12835-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription.
| ||
Nat Commun Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. | ||
Biomaterials Lysine demethylase KDM3A regulates nanophotonic hyperthermia resistance generated by 2D silicene in breast cancer. | ||
EMBO J Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity. | ||
Proc Natl Acad Sci U S A Histone demethylase JMJD1A promotes alternative splicing of AR variant 7 (AR-V7) in prostate cancer cells.
| ||
J Cell Biol KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.
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