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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KDM3A,JMJD1A Recombinant antibody, PBS Only

KDM3A,JMJD1A Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240532E2

Cat no : 83571-6-PBS

Synonyms

KDM3A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A, EC:1.14.11.65

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KDM3A,JMJD1A Recombinant antibody (83571-6-RR)

    IF Staining of HepG2 using 83571-6-RR (same clone as 83571-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KDM3A,JMJD1A antibody (83571-6-RR, Clone: 240532E2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83571-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using KDM3A,JMJD1A Recombinant antibody (83571-6-RR)

    FC experiment of HEK-293 using 83571-6-RR (same clone as 83571-6-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KDM3A,JMJD1A Recombinant antibody (83571-6-RR, Clone:240532E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83571-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83571-6-RR

    Affinity and Kinetic Characterization of 83571-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83571-6-RR against Human KDM3A,JMJD1A were performed. The affinity constant is 6.71 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83571-6-PBS is KDM3A,JMJD1A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KDM3A,JMJD1A fusion protein Ag28687 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 3A
Calculated molecular weight 150 kDa
GenBank accession numberBC038297
Gene symbol KDM3A/JMJD1A
Gene ID (NCBI) 55818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.