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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KDM3B Polyclonal antibody

KDM3B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19915-1-AP

Synonyms

JmjC domain-containing histone demethylation protein 2B, JHDM2B, EC:1.14.11.65, C5orf7, 5qNCA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KDM3B Polyclonal antibody (19915-1-AP)

    WB analysis using 19915-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19915-1-AP (KDM3B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using KDM3B Polyclonal antibody (19915-1-AP)

    WB analysis of human placenta using 19915-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19915-1-AP (KDM3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using KDM3B Polyclonal antibody (19915-1-AP)

    IF Staining of A431 using 19915-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KDM3B antibody (19915-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, HeLa cells, HepG2 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19915-1-AP targets KDM3B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KDM3B fusion protein Ag13795 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 3B
Calculated molecular weight 1761 aa, 192 kDa
Observed molecular weight 192 kDa, 85 kDa
GenBank accession numberBC001202
Gene symbol KDM3B
Gene ID (NCBI) 51780
RRIDAB_10693529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KDM3B antibody 19915-1-APDownload protocol
IF protocol for KDM3B antibody 19915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
View Article