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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KDM4A Polyclonal antibody (29977-1-AP)

    WB analysis using 29977-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using KDM4A Polyclonal antibody (29977-1-AP)

    WB analysis of mouse lung using 29977-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using KDM4A Polyclonal antibody (29977-1-AP)

    IP experiment of MCF-7 using 29977-1-AP

    IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with MCF-7 cells lysate 2120 ug.

  • Immunoprecipitation (IP) experiment of A549 cells using KDM4A Polyclonal antibody (29977-1-AP)

    IP experiment of A549 using 29977-1-AP

    IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with A549 cells lysate 800 ug.

  • Flow cytometry (FC) experiment of A549 cells using KDM4A Polyclonal antibody (29977-1-AP)

    FC experiment of A549 using 29977-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KDM4A (29977-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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KDM4A Polyclonal antibody

KDM4A Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 29977-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, ELISA

Synonyms

JMJD2, JmjC domain-containing histone demethylation protein 3A, JHDM3A, EC:1.14.11.66, [histone H3]-trimethyl-L-lysine(9) demethylase 4A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, mouse lung tissue, MCF-7 cells, MDA-MB-231 cells, NCl-H1299 cells
Positive IP detected inMCF-7 cells, A549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29977-1-AP targets KDM4A in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KDM4A fusion protein Ag31992 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 4A
Calculated molecular weight 1064 aa, 121 kDa
Observed molecular weight130-150 kDa
GenBank accession numberBC002558
Gene Symbol KDM4A
Gene ID (NCBI) 9682
RRIDAB_2923625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75164
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.

Protocols

Product Specific Protocols
WB protocol for KDM4A antibody 29977-1-APDownload protocol
IP protocol for KDM4A antibody 29977-1-APDownload protocol
FC protocol for KDM4A antibody 29977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols