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  • Featured Product
  • KD/KO Validated

KDM4D Polyclonal antibody

KDM4D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 22591-1-AP

Synonyms

JHDM3D, JMJD2D, KDM4D, lysine (K) demethylase 4D, Lysine specific demethylase 4D

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using KDM4D Polyclonal antibody (22591-1-AP)

    WB analysis of PC-3 using 22591-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22591-1-AP (KDM4D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using KDM4D Polyclonal antibody (22591-1-AP)

    WB analysis of PC-3 using 22591-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22591-1-AP (KDM4D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using KDM4D Polyclonal antibody (22591-1-AP)

    WB analysis of PC-3 using 22591-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22591-1-AP (KDM4D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thymus tissue using KDM4D Polyclonal antibody (22591-1-AP)

    IHC staining of human thymus using 22591-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22591-1-AP (KDM4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thymus tissue using KDM4D Polyclonal antibody (22591-1-AP)

    IHC staining of human thymus using 22591-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22591-1-AP (KDM4D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22591-1-AP targets KDM4D in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KDM4D fusion protein Ag18436 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 4D
Calculated molecular weight 523 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC122858
Gene symbol KDM4D
Gene ID (NCBI) 55693
RRIDAB_2879130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KDM4D antibody 22591-1-APDownload protocol
IHC protocol for KDM4D antibody 22591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IP

JHEP Rep

JMJD2D stabilises and cooperates with HBx protein to promote HBV transcription and replication

Authors - Xu Kong
  • KD Validated
View Article
ratChIP

Front Cell Dev Biol

A KDM4-DBC1-SIRT1 Axis Contributes to TGF-b Induced Mesenchymal Transition of Intestinal Epithelial Cells

Authors - Baoyu Chen
View Article