- Featured Product
- KD/KO Validated
RBP2 Polyclonal antibody
RBP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 18825-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse small intestine tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
18825-1-AP targets RBP2 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | lysine (K)-specific demethylase 5A |
| Calculated molecular weight | 192 kDa |
| Observed molecular weight | 192 kDa |
| GenBank accession number | NM_005056 |
| Gene symbol | KDM5A |
| Gene ID (NCBI) | 5927 |
| RRID | AB_10638436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBP2 antibody 18825-1-AP | Download protocol |
| IHC protocol for RBP2 antibody 18825-1-AP | Download protocol |
| IF protocol for RBP2 antibody 18825-1-AP | Download protocol |
| IP protocol for RBP2 antibody 18825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage | ||
Environ Toxicol JMJD3 downregulates IL4i1 aggravating lipopolysaccharide-induced acute lung injury via H3K27 and H3K4 demethylation | ||
Life Sci Treadmill exercise improves neurological function by inhibiting autophagy and the binding of HMGB1 to Beclin1 in MCAO juvenile rats. |
