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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VEGFR2/KDR Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP00012-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00012-3, Human VEGFR2/KDR Recombinant Matched Antibody Pair - PBS only. 83049-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0218.83049-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP00012-3

    Sandwich ELISA standard curve of MP00012-3, Human VEGFR2/KDR Recombinant Matched Antibody Pair - PBS only. 83049-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0218.83049-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Plasma of eight individual healthy human donors was measured. The human VEGFR2/KDR concentration of detected samples was determined to be 5,643.5 ng/mL with a range of 1,988.2 - 9,946.0 pg/mL.

    Sample test of MP00012-3

    Plasma of eight individual healthy human donors was measured. The human VEGFR2/KDR concentration of detected samples was determined to be 5,643.5 ng/mL with a range of 1,988.2 - 9,946.0 pg/mL.

  • HUVEC was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human VEGFR2/KDR, and measured 267.3 pg/mL.

    Sample test of MP00012-3

    HUVEC was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human VEGFR2/KDR, and measured 267.3 pg/mL.

  • The mean human VEGFR2/KDR concentration was determined to be 55,216.6 pg/mL in HUVEC cell extract based on a 0.4 mg/mL extract load.

    Sample test of MP00012-3

    The mean human VEGFR2/KDR concentration was determined to be 55,216.6 pg/mL in HUVEC cell extract based on a 0.4 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00012-3 targets VEGFR2/KDR in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: VEGFR2/KDR Recombinant antibody, PBS Only (Capture) 83049-2-PBS (230238D2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: VEGFR2/KDR Recombinant antibody, PBS Only (Detector) 83049-5-PBS (230238E8). 100 μg. Concentration 1 mgl/ml.

Alternative VEGFR2/KDR matched antibody pairs: MP00012-1, MP00012-2, MP00394-1, MP00394-2, MP00394-3, MP50080-1

Matched pair protein standard: Eg0218

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83049-2-PBS
Tested Reactivity human
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.15 x 10-9M
KOff=2.30 x 10-4M
KOn=2.00 x 105M
Full Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Synonyms KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1
GenBank accession numberNM_002253
Gene symbol KDR
UniprotP35968-1
Gene ID (NCBI) 3791
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83049-5-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Synonyms KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1
GenBank accession numberNM_002253
Gene symbol KDR
UniprotP35968-1
Gene ID (NCBI) 3791
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.