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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KEPI Polyclonal antibody (24933-1-AP)

    WB analysis using 24933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KEPI Polyclonal antibody (24933-1-AP)

    WB analysis using 24933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KEPI Polyclonal antibody (24933-1-AP)

    WB analysis of HeLa using 24933-1-AP

    WB result of KEPI antibody (24933-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEPI transfected HeLa cells.

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  • Featured Product
  • KD/KO Validated

KEPI Polyclonal antibody

KEPI Polyclonal Antibody for WB, ELISA
Cat No. 24933-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CPI17 like, KEPI, Kinase enhanced PP1 inhibitor, NY BR 81, PPP1R14C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24933-1-AP targets KEPI in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KEPI fusion protein Ag20645 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14C
Calculated molecular weight 18 kDa
Observed molecular weight20-22 kDa
GenBank accession numberBC015773
Gene Symbol PPP1R14C
Gene ID (NCBI) 81706
RRIDAB_3085762
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAE6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R14C, found as a morphine‐regulated brain gene, encodes protein phosphatase 1 regulatory inhibitor subunit 14C, and is identified as a potent inhibitor of PP1. Horvath and colleagues reported that PPP1R14C inhibited the release of neurotransmitters and promoted neuronal exocytosis by retaining synaptosome associated protein 25 (SNAP25) at a phosphorylated state. Moreover, overexpression of PPP1R14C increased the phosphorylation of RB transcriptional co‐repressor 1 (RB1) to protect leukemic cells from chemotherapy.

Protocols

Product Specific Protocols
WB protocol for KEPI antibody 24933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols