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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using KGA/GAC Monoclonal antibody (66265-1-Ig)

    WB analysis of NIH/3T3 using 66265-1-Ig (same clone as 66265-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using KGA/GAC Monoclonal antibody (66265-1-Ig)

    WB analysis of HEK-293 using 66265-1-Ig (same clone as 66265-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using KGA/GAC Monoclonal antibody (66265-1-Ig)

    WB analysis of rat liver using 66265-1-Ig (same clone as 66265-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using KGA/GAC Monoclonal antibody (66265-1-Ig)

    WB analysis of fetal human brain using 66265-1-Ig (same clone as 66265-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using KGA/GAC Monoclonal antibody (66265-1-Ig)

    WB analysis of U-251 using 66265-1-Ig (same clone as 66265-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KGA/GAC Monoclonal antibody (66265-1-Ig)

    IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KGA/GAC Monoclonal antibody (66265-1-Ig)

    IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KGA/GAC Monoclonal antibody (66265-1-Ig)

    IHC staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KGA/GAC Monoclonal antibody (66265-1-Ig)

    IHC staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KGA/GAC Monoclonal antibody (66265-1-Ig)

    IF Staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66265-1-Ig (GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KGA/GAC Monoclonal antibody (66265-1-Ig)

    IF Staining of HeLa using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KGA/GAC antibody (66265-1-Ig, Clone: 3A12A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KGA/GAC Monoclonal antibody (66265-1-Ig)

    IF Staining of HepG2 using 66265-1-Ig (same clone as 66265-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66265-1-Ig(GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KGA/GAC Monoclonal antibody, PBS Only

KGA/GAC Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66265-1-PBS
Clone No.3A12A1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

GLS, KGA, GAM, GAC, EC:3.5.1.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66265-1-PBS targets KGA/GAC in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen KGA/GAC fusion protein Ag24227 相同性解析による交差性が予測される生物種
Full Name glutaminase
Calculated molecular weight 669 aa, 73 kDa
Observed molecular weight 58 kDa, 65 kDa
GenBank accession numberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
RRIDAB_2881651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94925
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.