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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KGA-Specific Monoclonal antibody, PBS Only

KGA-Specific Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11A11

Cat no : 66265-2-PBS

Synonyms

GLS, KGA, GAM, GAC, EC:3.5.1.2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using KGA-Specific Monoclonal antibody (66265-2-Ig)

    WB analysis of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using KGA-Specific Monoclonal antibody (66265-2-Ig)

    WB analysis of HEK-293 using 66265-2-Ig (same clone as 66265-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using KGA-Specific Monoclonal antibody (66265-2-Ig)

    WB analysis of COLO 320 using 66265-2-Ig (same clone as 66265-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using KGA-Specific Monoclonal antibody (66265-2-Ig)

    WB analysis of HSC-T6 using 66265-2-Ig (same clone as 66265-2-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using KGA-Specific Monoclonal antibody (66265-2-Ig)

    WB analysis of NIH/3T3 using 66265-2-Ig (same clone as 66265-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KGA-Specific Monoclonal antibody (66265-2-Ig)

    IHC staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-2-Ig (GLS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KGA-Specific Monoclonal antibody (66265-2-Ig)

    IHC staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-2-Ig (GLS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KGA-Specific Monoclonal antibody (66265-2-Ig)

    IF Staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KGA-Specific antibody (66265-2-Ig, Clone: 1B11A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KGA-Specific Monoclonal antibody (66265-2-Ig)

    IF Staining of mouse brain using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KGA-Specific antibody (66265-2-Ig, Clone: 1B11A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using KGA-Specific Monoclonal antibody (66265-2-Ig)

    IF Staining of A549 using 66265-2-Ig (same clone as 66265-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using KGA-Specific antibody (66265-2-Ig, Clone: 1B11A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66265-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66265-2-PBS targets KGA-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KGA-Specific fusion protein Ag24227 相同性解析による交差性が予測される生物種
Full Name glutaminase
Calculated molecular weight 669 aa, 73 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC038507
Gene symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.