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Sam68 Polyclonal antibody

Name: Sam68 Polyclonal antibody
Brand: Proteintech
SKU: 10222-1-AP
Rating: 3.00 (1 reviews)

Sam68 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 10222-1-AP

Synonyms

KHDRBS1, Src-associated in mitosis 68 kDa protein, p21 Ras GTPase-activating protein-associated p62, KHDRBS 1, KH domain-containing, RNA-binding, signal transduction-associated protein 1

Validation Data Gallery

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AllWBIPIHCIF-P

WB analysis using 10222-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 10222-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10222-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10222-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10222-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10222-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10222-1-AP
IP result of anti-Sam68 (IP:10222-1-AP, 4ug; Detection:10222-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human breast cancer using 10222-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10222-1-AP (KHDRBS1 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of mouse brain using 10222-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10222-1-AP (Sam68 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10222-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HT-29 cells, mouse brain tissue, NIH/3T3 cells, mouse embryo tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below
IHC	See 4 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

10222-1-AP targets Sam68 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Sam68 fusion protein Ag0235 相同性解析による交差性が予測される生物種
Full Name	KH domain containing, RNA binding, signal transduction associated 1
Calculated molecular weight	48 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC000717
Gene symbol	Sam68
Gene ID (NCBI)	10657
RRID	AB_2130292
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and INS receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. KHDRBS1 has three isoforms with calculated MW of 44-48kd. For isoform with a calculated MW of 48.2 kD, it migrated as a 68kD protein on SDS-PAGE gels[PMID: 10564820]. This ia a rabbit polyclonal antibody raised against residues near the N terminus of human KHDRBS1.

Protocols

Product Specific Protocols
WB protocol for Sam68 antibody 10222-1-AP	Download protocol
IHC protocol for Sam68 antibody 10222-1-AP	Download protocol
IF protocol for Sam68 antibody 10222-1-AP	Download protocol
IP protocol for Sam68 antibody 10222-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Acta Neuropathol Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations. Authors - Neumann Manuela M View Article
mouse	IHC	Brain Regulatory T cells delay disease progression in Alzheimer-like pathology. Authors - Cira Dansokho View Article
human	IHC	Theranostics SAM68 promotes tumorigenesis in lung adenocarcinoma by regulating metabolic conversion via PKM alternative splicing. Authors - Song Zhu View Article
human	WB,CoIP,RIP	Mol Ther Nucleic Acids LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation Authors - Jianbo Feng View Article
human	WB	FASEB J CircMTA1 promotes glioblastoma angiogenesis by encoding MTA1-134aa Authors - Ben Niu View Article
mouse	WB	Reprod Fertil Dev Cold-induced RNA-binding protein (CIRBP) regulates the expression of Src-associated during mitosis of 68 kDa (Sam68) and extracellular signal-regulated kinases (ERK) during heat stress-induced testicular injury. Authors - Chengcheng Xu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Julia (Verified Customer) (08-07-2024)

Used for 30ug of mESC. Many bands are visible with the thickest one at the correct size. Increasing the dilution should help to obtain cleaner picture.

Applications: Western Blot
Primary Antibody Dilution: 1:3000
Cell Tissue Type: mESC

Sam68 Antibody Western Blot validation (1:3000 dilution) in mESC (Cat no:10222-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Sam68 Polyclonal antibody

Sam68 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 10222-1-AP

WB analysis using 10222-1-AP

WB analysis of NIH/3T3 using 10222-1-AP

WB analysis of mouse brain using 10222-1-AP

WB analysis of HeLa using 10222-1-AP

WB analysis of HEK-293 using 10222-1-AP

IP experiment of HeLa using 10222-1-AP

IHC staining of human breast cancer using 10222-1-AP

IF Staining of mouse brain using 10222-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Regulatory T cells delay disease progression in Alzheimer-like pathology.

SAM68 promotes tumorigenesis in lung adenocarcinoma by regulating metabolic conversion via PKM alternative splicing.

LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation

CircMTA1 promotes glioblastoma angiogenesis by encoding MTA1-134aa

Cold-induced RNA-binding protein (CIRBP) regulates the expression of Src-associated during mitosis of 68 kDa (Sam68) and extracellular signal-regulated kinases (ERK) during heat stress-induced testicular injury.

Reviews