Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IF | See 2 publications below |
| RIP | See 1 publications below |
Product Information
55409-1-AP targets KHSRP in WB, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | KH-type splicing regulatory protein |
| Calculated molecular weight | 73 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | NM_003685 |
| Gene Symbol | KHSRP |
| Gene ID (NCBI) | 8570 |
| RRID | AB_11182170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92945 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KHSRP antibody 55409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities. | ||
iScience Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1 | ||
Atherosclerosis microRNA-185 modulates low density lipoprotein receptor expression as a key posttranscriptional regulator. | ||
Int Immunopharmacol Angiotensin II contributes to intratumoral immunosuppressionvia induction of PD-L1 expression in non-small cell lung carcinoma.
| ||
Cell Rep Identification of cis-HOX-HOXC10 axis as a therapeutic target for colorectal tumor-initiating cells without APC mutations.
| ||
Biochim Biophys Acta Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection. |