Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, mouse spleen tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82931-3-RR targets KHSRP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | KHSRP fusion protein Ag33890 相同性解析による交差性が予測される生物種 |
Full Name | KH-type splicing regulatory protein |
Calculated molecular weight | 73 kDa |
Observed molecular weight | 75 kDa |
GenBank accession number | NM_003685 |
Gene Symbol | KHSRP |
Gene ID (NCBI) | 8570 |
RRID | AB_3670671 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q92945 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Protocols
Product Specific Protocols | |
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WB protocol for KHSRP antibody 82931-3-RR | Download protocol |
IHC protocol for KHSRP antibody 82931-3-RR | Download protocol |
IF protocol for KHSRP antibody 82931-3-RR | Download protocol |
IP protocol for KHSRP antibody 82931-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |