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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ki-67 Recombinant antibody, PBS Only

Ki-67 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241499B1

Cat no : 84192-3-PBS

Synonyms

MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using Ki-67 Recombinant antibody (84192-3-RR)

    WB analysis of MCF-7 using 84192-3-RR (same clone as 84192-3-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84192-3-RR (Ki-67 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human placenta using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human placenta using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human malignant melanoma using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human tonsillitis using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human colon cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human colon cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human malignant melanoma using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human ovarian cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using Ki-67 Recombinant antibody (84192-3-RR)

    IHC staining of human ovarian cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of human lung cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of human thyroid cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of human thyroid cancer using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of HeLa using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of A549 using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of A431 using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of U2OS using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of MCF-7 using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of hTERT-RPE1 using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ki-67 Recombinant antibody (84192-3-RR)

    IF Staining of HepG2 using 84192-3-RR (same clone as 84192-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84192-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84192-3-PBS targets Ki-67 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki-67
Calculated molecular weight 359 kDa
GenBank accession numberNM_002417
Gene symbol KI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.