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  • Featured Product
  • KD/KO Validated

KIAA0182 Polyclonal antibody, PBS Only

KIAA0182 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24947-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA0182 Polyclonal antibody (24947-1-AP)

    WB analysis using 24947-1-AP (same clone as 24947-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24947-1-AP (KIAA0182 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of mouse testis using 24947-1-AP (same clone as 24947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of human kidney using 24947-1-AP (same clone as 24947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of mouse testis using 24947-1-AP (same clone as 24947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of human kidney using 24947-1-AP (same clone as 24947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KIAA0182 Polyclonal antibody (24947-1-AP)

    IF Staining of U2OS using 24947-1-AP (same clone as 24947-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIAA0182 antibody (24947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24947-1-PBS targets KIAA0182 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA0182 fusion protein Ag20674 相同性解析による交差性が予測される生物種
Full Name KIAA0182
Calculated molecular weight 1217 aa, 136 kDa
Observed molecular weight 136 kDa
GenBank accession numberBC037556
Gene symbol KIAA0182
Gene ID (NCBI) 23199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.