Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68717-1-Ig targets KIAA0319L in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KIAA0319L fusion protein Ag15263 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA0319-like |
| Calculated molecular weight | 1049 aa, 116 kDa |
| Observed molecular weight | 140-150 kDa |
| GenBank accession number | BC031672 |
| Gene Symbol | KIAA0319L |
| Gene ID (NCBI) | 79932 |
| RRID | AB_3670410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IZA0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA0319L antibody 68717-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |