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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA0368 Polyclonal antibody

KIAA0368 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25684-1-AP

Synonyms

ECM29, extracellular mutant 29, KIAA0368, KIAA1962, RP11 386D8.2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KIAA0368 Polyclonal antibody (25684-1-AP)

    WB analysis of HEK-293 using 25684-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25684-1-AP (KIAA0368 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using KIAA0368 Polyclonal antibody (25684-1-AP)

    WB analysis of A549 using 25684-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25684-1-AP (KIAA0368 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using KIAA0368 Polyclonal antibody (25684-1-AP)

    IP experiment of HEK-293 using 25684-1-AP

    IP result of anti-KIAA0368 (IP:25684-1-AP, 4ug; Detection:25684-1-AP 1:600) with HEK-293 cells lysate 2600ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using KIAA0368 Polyclonal antibody (25684-1-AP)

    IHC staining of human liver using 25684-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25684-1-AP (KIAA0368 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using KIAA0368 Polyclonal antibody (25684-1-AP)

    IHC staining of human liver using 25684-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25684-1-AP (KIAA0368 antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using KIAA0368 Polyclonal antibody (25684-1-AP)

    IF Staining of HEK-293 using 25684-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25684-1-AP (KIAA0368 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, A549 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 25684-1-AP is KIAA0368 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA0368 fusion protein Ag22309 相同性解析による交差性が予測される生物種
Full Name KIAA0368
Calculated molecular weight 1845 aa, 204 kDa
Observed molecular weight 205 kDa
GenBank accession numberBC021127
Gene symbol KIAA0368
Gene ID (NCBI) 23392
RRIDAB_2880193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ecm29 binds specifically to 26 S proteasomes. Ecm29 has been reported to stabilize proteasomes, to remodel proteasomes upon stress, to be involved in membrane-associated localization of proteasomes, and, more recently, to be involved in quality control or assembly of the proteasome. Ecm29 acts as a proteasome inhibitor.

Protocols

Product Specific Protocols
WB protocol for KIAA0368 antibody 25684-1-APDownload protocol
IHC protocol for KIAA0368 antibody 25684-1-APDownload protocol
IF protocol for KIAA0368 antibody 25684-1-APDownload protocol
IP protocol for KIAA0368 antibody 25684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nanomicro Lett

Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling

Authors - Junji Ren
View Article