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KD/KO Validated

ATG13 Polyclonal antibody

Name: ATG13 Polyclonal antibody
Brand: Proteintech
SKU: 18258-1-AP

ATG13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18258-1-AP

Synonyms

ATG13, Autophagy related protein 13, KIAA0652

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of SH-SY5Y using 18258-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 18258-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 18258-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18258-1-AP
WB result of ATG13 antibody (18258-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG13 transfected HeLa cells.
WB analysis using 18258-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of BGC-823 using 18258-1-AP
BGC-823 cells were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 18258-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 18258-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 18258-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 18258-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 18258-1-AP
IP result of anti-ATG13 (IP:18258-1-AP, 4ug; Detection:18258-1-AP 1:300) with SH-SY5Y cells lysate 1600ug.
IHC staining of mouse heart using 18258-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 18258-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18258-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18258-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 18258-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 18258-1-AP (ATG13 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 18258-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 18258-1-AP (ATG13 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of SH-SY5Y using 18258-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 18258-1-AP (ATG13 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 18258-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SH-SY5Y cells, BGC-823 cells, HeLa cells, human brain tissue, mouse cerebellum tissue, mouse testis tissue, mouse thymus tissue, Jurkat cells, HEK-293 cells, MCF-7 cells
Positive IP detected in	SH-SY5Y cells
Positive IHC detected in	mouse heart tissue, mouse brain tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 17 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

18258-1-AP targets ATG13 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATG13 fusion protein Ag13090 相同性解析による交差性が予測される生物種
Full Name	KIAA0652
Calculated molecular weight	57 kDa
Observed molecular weight	57-63 kDa
GenBank accession number	BC001331
Gene symbol	ATG13
Gene ID (NCBI)	9776
RRID	AB_2130658
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Protocols

Product Specific Protocols
WB protocol for ATG13 antibody 18258-1-AP	Download protocol
IHC protocol for ATG13 antibody 18258-1-AP	Download protocol
IF protocol for ATG13 antibody 18258-1-AP	Download protocol
IP protocol for ATG13 antibody 18258-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Noncanonical autophagy promotes the visual cycle. Authors - Kim Ji-Young JY KD Validated View Article
human	WB	J Clin Invest p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease. Authors - Zhengwei Ma View Article
mouse		Autophagy The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy. Authors - Wan Yun Ho View Article
human	WB	Oxid Med Cell Longev Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway Authors - Ruyuan Wei View Article
mouse	WB	Stem Cell Res Ther Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis. Authors - Caixia Jin View Article
human	WB	Ecotoxicol Environ Saf Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain. Authors - Hongmei Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG13 Polyclonal antibody

ATG13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of SH-SY5Y using 18258-1-AP

WB analysis using 18258-1-AP

WB analysis using 18258-1-AP

WB analysis of HeLa using 18258-1-AP

WB analysis using 18258-1-AP

WB analysis of BGC-823 using 18258-1-AP

WB analysis of mouse thymus using 18258-1-AP

WB analysis of human brain using 18258-1-AP

WB analysis of mouse cerebellum using 18258-1-AP

WB analysis of mouse testis using 18258-1-AP

IP experiment of SH-SY5Y using 18258-1-AP

IHC staining of mouse heart using 18258-1-AP

IHC staining of mouse heart using 18258-1-AP

IHC staining of mouse brain using 18258-1-AP

IHC staining of mouse brain using 18258-1-AP

IHC staining of human brain using 18258-1-AP

IHC staining of human brain using 18258-1-AP

IF Staining of SH-SY5Y using 18258-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Noncanonical autophagy promotes the visual cycle.

p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease.

The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy.

Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway

Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.

Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain.