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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA0774 Polyclonal antibody

KIAA0774 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24921-1-AP

Synonyms

TIP150, MTUS2, Microtubule-associated tumor suppressor candidate 2, Microtubule plus-end tracking protein TIP150, CAZIP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using KIAA0774 Polyclonal antibody (24921-1-AP)

    WB analysis of mouse brain using 24921-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24921-1-AP (KIAA0774 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using KIAA0774 Polyclonal antibody (24921-1-AP)

    WB analysis of mouse heart using 24921-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24921-1-AP (KIAA0774 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using KIAA0774 Polyclonal antibody (24921-1-AP)

    IHC staining of human heart using 24921-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24921-1-AP (KIAA0774 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using KIAA0774 Polyclonal antibody (24921-1-AP)

    IHC staining of human heart using 24921-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24921-1-AP (KIAA0774 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIAA0774 Polyclonal antibody (24921-1-AP)

    IF Staining of HepG2 using 24921-1-AP

    Immunofluorescent analysis of HepG2 cells using 24921-1-AP (KIAA0774 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24921-1-AP targets KIAA0774 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA0774 fusion protein Ag20593 相同性解析による交差性が予測される生物種
Full Name KIAA0774
Calculated molecular weight 1369 aa, 150 kDa
Observed molecular weight 150 kDa, 41 kDa
GenBank accession numberBC032481
Gene symbol KIAA0774
Gene ID (NCBI) 23281
RRIDAB_2879797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA0774, also named as MTUS2, CAZIP and TIP150, binds microtubules. It may regulate the dynamics of microtubules at their growing distal tip. KIAA0774 has three isoforms with MW 150 kDa, 41 kDa and 30 kDa.

Protocols

Product Specific Protocols
WB protocol for KIAA0774 antibody 24921-1-APDownload protocol
IHC protocol for KIAA0774 antibody 24921-1-APDownload protocol
IF protocol for KIAA0774 antibody 24921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols