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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA0776 Recombinant antibody

KIAA0776 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230363G7

Cat no : 83047-5-RR

Synonyms

MAXER, EC:2.3.2.-, E3 UFM1-protein transferase 1, E3 UFM1-protein ligase 1, E3 UFM1 protein ligase 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIAA0776 Recombinant antibody (83047-5-RR)

    IF Staining of HepG2 using 83047-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIAA0776 antibody (83047-5-RR, Clone: 230363G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of U2OS cells using KIAA0776 Recombinant antibody (83047-5-RR)

    FC experiment of U2OS using 83047-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human KIAA0776 (83047-5-RR, Clone:230363G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83047-5-RR

    Affinity and Kinetic Characterization of 83047-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83047-5-RR against Human KIAA0776 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83047-5-RR targets KIAA0776 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIAA0776 fusion protein Ag23362 相同性解析による交差性が予測される生物種
Full Name KIAA0776
GenBank accession numberBC036379
Gene symbol KIAA0776
Gene ID (NCBI) 23376
RRIDAB_3670773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for KIAA0776 antibody 83047-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols