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KIAA1199 / CEMIP Polyclonal antibody
KIAA1199 / CEMIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 21129-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U-87 MG cells, U2OS cells, COLO 320 cells, SGC-7901 cells |
| Positive IHC detected in | human prostate hyperplasia tissue, mouse testis tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 16 publications below |
| WB | See 31 publications below |
| IHC | See 27 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
21129-1-AP targets KIAA1199 / CEMIP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, marmoset |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KIAA1199 / CEMIP fusion protein Ag15527 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1199 |
| Calculated molecular weight | 1361 aa, 153 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC020256 |
| Gene symbol | KIAA1199 |
| Gene ID (NCBI) | 57214 |
| RRID | AB_10695771 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA1199 / CEMIP antibody 21129-1-AP | Download protocol |
| IHC protocol for KIAA1199 / CEMIP antibody 21129-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun TREX tetramer disruption alters RNA processing necessary for corticogenesis in THOC6 Intellectual Disability Syndrome | ||
Sci Adv Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment. | ||
Am J Hum Genet Altered regulation of DPF3, a member of the SWI/SNF complexes, underlies the 14q24 renal cancer susceptibility locus.
| ||
Theranostics Functional Genomic mRNA Profiling of Colorectal Adenomas: Identification and in vivo Validation of CD44 and Splice Variant CD44v6 as Molecular Imaging Targets. | ||
Cell Rep Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. | ||
Oncogene KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH DAN (Verified Customer) (06-10-2022) |
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