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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA1199 / CEMIP Polyclonal antibody

KIAA1199 / CEMIP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31106-1-AP

Synonyms

KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1199 / CEMIP Polyclonal antibody (31106-1-AP)

    WB analysis using 31106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31106-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KIAA1199 / CEMIP Polyclonal antibody (31106-1-AP)

    IHC staining of human colon cancer using 31106-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 31106-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHCT 116 cells, HT-1080 cells
Positive IHC detected inhuman colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31106-1-AP targets KIAA1199 / CEMIP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1199 / CEMIP fusion protein Ag34315 相同性解析による交差性が予測される生物種
Full Name KIAA1199 / CEMIP
Calculated molecular weight 1361 aa, 153 kDa
Observed molecular weight150 kDa
GenBank accession numberBC020256
Gene symbol KIAA1199
Gene ID (NCBI) 57214
RRIDAB_3669858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

Protocols

Product Specific Protocols
WB protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
IHC protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols