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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1217 Recombinant antibody (83361-2-RR)

    WB analysis using 83361-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83361-2-RR (KIAA1217 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KIAA1217 Recombinant antibody (83361-2-RR)

    WB analysis of HEK-293 using 83361-2-RR

    WB result of IAA1217' target='_blank'>KIAA1217 antibody (83361-2-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IAA1217' target='_blank'>KIAA1217 transfected HEK-293 cells.

  • Flow cytometry (FC) experiment of MCF-7 cells using KIAA1217 Recombinant antibody (83361-2-RR)

    FC experiment of MCF-7 using 83361-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KIAA1217 Recombinant antibody (83361-2-RR, Clone:240276F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83361-2-RR

    Affinity and Kinetic Characterization of 83361-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83361-2-RR against Human KIAA1217 were performed. The affinity constant is 1.46 nM.

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  • Featured Product
  • KD/KO Validated

KIAA1217 Recombinant antibody

KIAA1217 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83361-2-RR
Clone No.240276F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

SKT, Sickle tail protein homolog, 240276F3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83361-2-RR targets KIAA1217 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIAA1217 fusion protein Ag19367 相同性解析による交差性が予測される生物種
Full Name KIAA1217
Calculated molecular weight 1943 aa, 214 kDa
Observed molecular weight100-110, 140-160, 210
GenBank accession numberBC018764
Gene Symbol KIAA1217
Gene ID (NCBI) 56243
RRIDAB_3671015
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5T5P2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.

Protocols

Product Specific Protocols
WB protocol for KIAA1217 antibody 83361-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols