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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1279 Polyclonal antibody (25653-1-AP)

    WB analysis using 25653-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using KIAA1279 Polyclonal antibody (25653-1-AP)

    WB analysis of mouse kidney using 25653-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KIAA1279 Polyclonal antibody (25653-1-AP)

    WB analysis of HEK-293 using 25653-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KIAA1279 Polyclonal antibody (25653-1-AP)

    IHC staining of mouse kidney using 25653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIAA1279 Polyclonal antibody (25653-1-AP)

    IHC staining of mouse testis using 25653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIAA1279 Polyclonal antibody (25653-1-AP)

    IHC staining of mouse testis using 25653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KIAA1279 Polyclonal antibody (25653-1-AP)

    IHC staining of mouse kidney using 25653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

KIAA1279 Polyclonal antibody

KIAA1279 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25653-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

KBP, KIAA1279, KIF1 binding protein, TTC20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, NIH/3T3 cells
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25653-1-AP targets KIAA1279 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1279 fusion protein Ag22234 相同性解析による交差性が予測される生物種
Full Name KIAA1279
Calculated molecular weight 621 aa, 72 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC012180
Gene Symbol KIAA1279
Gene ID (NCBI) 26128
RRIDAB_2880178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KIAA1279 antibody 25653-1-APDownload protocol
IHC protocol for KIAA1279 antibody 25653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Mol Neurosci

Multiple Functions of KBP in Neural Development Underlie Brain Anomalies in Goldberg-Shprintzen Syndrome.

Authors - Hsin-Yun Chang
  • KD Validated
View Article
mouseWB, IF

Reproduction

KIF1-binding protein interacts with KIF3A in haploid male germ cells.

Authors - Mari S Lehti
View Article