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  • Featured Product
  • KD/KO Validated

VIRMA/KIAA1429 Polyclonal antibody

VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30166-1-AP

Synonyms

KIAA1429, VIRMA, MSTP054

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1429 Polyclonal antibody (30166-1-AP)

    WB analysis using 30166-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VIRMA/KIAA1429 Polyclonal antibody (30166-1-AP)

    IHC staining of mouse kidney using 30166-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using VIRMA/KIAA1429 Polyclonal antibody (30166-1-AP)

    IF Staining of U2OS using 30166-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using VIRMA/KIAA1429 antibody (30166-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, K-562 cells, MDA-MB-231 cells, SKOV-3 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30166-1-AP targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VIRMA/KIAA1429 fusion protein Ag32830 相同性解析による交差性が予測される生物種
Full Name KIAA1429
Observed molecular weight200-210 kDa
GenBank accession numberBC003701
Gene symbol KIAA1429
Gene ID (NCBI) 25962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. Moreover, this antibody can not recognize short isoforms.

Protocols

Product Specific Protocols
WB protocol for VIRMA/KIAA1429 antibody 30166-1-APDownload protocol
IHC protocol for VIRMA/KIAA1429 antibody 30166-1-APDownload protocol
IF protocol for VIRMA/KIAA1429 antibody 30166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

KIAA1429 facilitates metastasis via m6A-YTHDC1-dependent RND3 down-regulation in hepatocellular carcinoma cells

Authors - Meihua Shan
  • KD Validated
View Article