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- KD/KO Validated
VIRMA/KIAA1429 Polyclonal antibody
VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 30166-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, K-562 cells, MDA-MB-231 cells, SKOV-3 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
30166-1-AP targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VIRMA/KIAA1429 fusion protein Ag32830 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1429 |
| Observed molecular weight | 200-210 kDa |
| GenBank accession number | BC003701 |
| Gene symbol | KIAA1429 |
| Gene ID (NCBI) | 25962 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. Moreover, this antibody can not recognize short isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VIRMA/KIAA1429 antibody 30166-1-AP | Download protocol |
| IHC protocol for VIRMA/KIAA1429 antibody 30166-1-AP | Download protocol |
| IF protocol for VIRMA/KIAA1429 antibody 30166-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett KIAA1429 facilitates metastasis via m6A-YTHDC1-dependent RND3 down-regulation in hepatocellular carcinoma cells
| ||
BMC Biol The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis |
