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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CIP2A Monoclonal antibody

CIP2A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A3F3

Cat no : 67843-1-Ig

Synonyms

Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, p90, p90 autoantigen, Protein CIP2A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CIP2A Monoclonal antibody (67843-1-Ig)

    WB analysis using 67843-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using CIP2A Monoclonal antibody (67843-1-Ig)

    WB analysis of HSC-T6 using 67843-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using CIP2A Monoclonal antibody (67843-1-Ig)

    WB analysis of PC-12 using 67843-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CIP2A Monoclonal antibody (67843-1-Ig)

    IF Staining of HeLa using 67843-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KIAA1524 antibody (67843-1-Ig, Clone: 2A3F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67843-1-Ig targets CIP2A in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CIP2A fusion protein Ag19598 相同性解析による交差性が予測される生物種
Full Name KIAA1524
Calculated molecular weight 905 aa, 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC130564
Gene symbol CIP2A
Gene ID (NCBI) 57650
RRIDAB_2918605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)

Protocols

Product Specific Protocols
WB protocol for CIP2A antibody 67843-1-IgDownload protocol
IF protocol for CIP2A antibody 67843-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols