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Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using Shootin 1 Polyclonal antibody (24383-1-AP)

    WB analysis of Neuro-2a using 24383-1-AP

    WB result of Shootin 1 antibody (24383-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Shootin 1 transfected Neuro-2a cells.

  • Western Blot (WB) analysis of various lysates using Shootin 1 Polyclonal antibody (24383-1-AP)

    WB analysis using 24383-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24383-1-AP (Shootin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using Shootin 1 Polyclonal antibody (24383-1-AP)

    WB analysis of rat brain using 24383-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 24383-1-AP (KIAA1598 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using Shootin 1 Polyclonal antibody (24383-1-AP)

    IHC staining of human bowen disease using 24383-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24383-1-AP (Shootin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease tissue using Shootin 1 Polyclonal antibody (24383-1-AP)

    IHC staining of human bowen disease using 24383-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24383-1-AP (Shootin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Shootin 1 Polyclonal antibody

Shootin 1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24383-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SHTN1, KIAA1598, Shootin-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, Neuro-2a cells, rat brain tissue, SK-N-SH cells
Positive IHC detected inhuman bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24383-1-AP targets Shootin 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Shootin 1 fusion protein Ag18294 相同性解析による交差性が予測される生物種
Full Name KIAA1598
Calculated molecular weight 72 kDa
Observed molecular weight 60-75 kDa
GenBank accession numberBC022348
Gene Symbol Shootin 1
Gene ID (NCBI) 57698
RRIDAB_2879517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA0MZ66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Shootin 1 antibody 24383-1-APDownload protocol
IHC protocol for Shootin 1 antibody 24383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Genet

Data-mining-based biomarker evaluation and experimental validation of SHTN1 for bladder cancer

Authors - Yueying Wang
View Article