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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EPG5 Polyclonal antibody

EPG5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27666-1-AP

Synonyms

EPG5, HEEW1, KIAA1632, UPF0493 protein KIAA1632

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPG5 Polyclonal antibody (27666-1-AP)

    WB analysis using 27666-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27666-1-AP (EPG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EPG5 Polyclonal antibody (27666-1-AP)

    IHC staining of human kidney using 27666-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using EPG5 Polyclonal antibody (27666-1-AP)

    IHC staining of human kidney using 27666-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27666-1-AP targets EPG5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPG5 fusion protein Ag24801 相同性解析による交差性が予測される生物種
Full Name KIAA1632
Calculated molecular weight 2579 aa, 292 kDa
Observed molecular weight270-290 kDa
GenBank accession numberBC130614
Gene symbol EPG5
Gene ID (NCBI) 57724
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPG5, a metazoan-specific autophagy protein, regulates autophagy by promoting fusion of autophagosomes with lysosomes and/or late endosomes. EPG5 regulates the stability and the assembly of STX17-SNAP29-VAMP8 trans-SNARE complexes. Loss of function of EPG5 causes non-specific fusion of autophagosomes with other endocytic vesicles, resulting in formation of non-degradative enlarged vesicles. EPG5 expression in human pluripotent stem cells like ESC and iPSC is higher than that of human somatic cells.(PMID: 30931944, PMID: 27588602)

Protocols

Product Specific Protocols
WB protocol for EPG5 antibody 27666-1-APDownload protocol
IHC protocol for EPG5 antibody 27666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols