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CEP44 Polyclonal antibody
CEP44 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 24457-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
Product Information
24457-1-AP targets CEP44 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CEP44 fusion protein Ag19961 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1712 |
| Calculated molecular weight | 390 aa, 44 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | BC038667 |
| Gene symbol | CEP44 |
| Gene ID (NCBI) | 80817 |
| RRID | AB_2879557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CEP44, also named as KIAA1712 and PS1TP3, localizes to centrosomes, spindle poles, and weakly in midbody. It is a 44 kDa centrosome protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP44 antibody 24457-1-AP | Download protocol |
| IHC protocol for CEP44 antibody 24457-1-AP | Download protocol |
| IF protocol for CEP44 antibody 24457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning | ||
