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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA1715 Polyclonal antibody

KIAA1715 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30857-1-AP

Synonyms

DKFZp686G1442, KIAA1715, LNP, Protein lunapark, Ul, ulnaless

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1715 Polyclonal antibody (30857-1-AP)

    WB analysis using 30857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30857-1-AP (KIAA1715 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KIAA1715 Polyclonal antibody (30857-1-AP)

    IHC staining of mouse cerebellum using 30857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30857-1-AP (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30857-1-AP targets KIAA1715 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1715 fusion protein Ag33996 相同性解析による交差性が予測される生物種
Full Name KIAA1715
Observed molecular weight53 kDa
GenBank accession numberBC105132
Gene symbol KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3086424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

Protocols

Product Specific Protocols
WB protocol for KIAA1715 antibody 30857-1-APDownload protocol
IHC protocol for KIAA1715 antibody 30857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols