• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA1715 Polyclonal antibody

KIAA1715 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31308-1-AP

Synonyms

LNP, ER junction formation factor lunapark, ulnaless, Ul, Protein lunapark

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using KIAA1715 Polyclonal antibody (31308-1-AP)

    WB analysis using 31308-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 31308-1-AP (KIAA1715 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KIAA1715 Polyclonal antibody (31308-1-AP)

    IHC staining of mouse cerebellum using 31308-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31308-1-AP (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KIAA1715 Polyclonal antibody (31308-1-AP)

    IF Staining of U2OS using 31308-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIAA1715 antibody (31308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inU2OS cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31308-1-AP targets KIAA1715 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1715 fusion protein Ag34669 相同性解析による交差性が予測される生物種
Full Name KIAA1715
Observed molecular weight51 kDa
GenBank accession numberBC105132
Gene symbol KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3669942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

Protocols

Product Specific Protocols
WB protocol for KIAA1715 antibody 31308-1-APDownload protocol
IHC protocol for KIAA1715 antibody 31308-1-APDownload protocol
IF protocol for KIAA1715 antibody 31308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols