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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1715 Recombinant antibody (83418-5-RR)

    WB analysis using 83418-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83418-5-RR (KIAA1715 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KIAA1715 Recombinant antibody (83418-5-RR)

    IHC staining of mouse cerebellum using 83418-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-5-RR (KIAA1715 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using KIAA1715 Recombinant antibody (83418-5-RR)

    IF Staining of U-251 using 83418-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using KIAA1715 antibody (83418-5-RR, Clone: 240211B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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KIAA1715 Recombinant antibody

KIAA1715 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83418-5-RR
Clone No.240211B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HepG2 cells, U2OS cells, mouse testis tissue, mouse colon tissue, mouse kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83418-5-RR targets KIAA1715 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIAA1715 fusion protein Ag34669 相同性解析による交差性が予測される生物種
Full Name KIAA1715
Observed molecular weight53-55 kDa
GenBank accession numberBC105132
Gene Symbol KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3671067
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9C0E8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

Protocols

Product Specific Protocols
WB protocol for KIAA1715 antibody 83418-5-RRDownload protocol
IHC protocol for KIAA1715 antibody 83418-5-RRDownload protocol
IF protocol for KIAA1715 antibody 83418-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols