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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA2013 Polyclonal antibody

KIAA2013 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27886-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KIAA2013 Polyclonal antibody (27886-1-AP)

    WB analysis of HEK-293 using 27886-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27886-1-AP (KIAA2013 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KIAA2013 Polyclonal antibody (27886-1-AP)

    WB analysis using 27886-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27886-1-AP (KIAA2013 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using KIAA2013 Polyclonal antibody (27886-1-AP)

    WB analysis of U2OS using 27886-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 27886-1-AP (KIAA2013 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KIAA2013 Polyclonal antibody (27886-1-AP)

    IHC staining of human ovary cancer using 27886-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27886-1-AP (KIAA2013 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KIAA2013 Polyclonal antibody (27886-1-AP)

    IHC staining of human ovary cancer using 27886-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27886-1-AP (KIAA2013 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, U2OS cells, mouse kidney tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27886-1-AP targets KIAA2013 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA2013 fusion protein Ag27379 相同性解析による交差性が予測される生物種
Full Name KIAA2013
Observed molecular weight 65 kDa
GenBank accession numberBC035033
Gene symbol KIAA2013
Gene ID (NCBI) 90231
RRIDAB_2881004
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA2013, also known as Q8IYS2 or MGC33867, is a single-pass transmembrane protein encoded by the KIAA2013 gene in humans. KIAA2013 has been found to play a role in the Endocannabinoid system. This system is made up of cannabinoid receptors 1 and 2 (CB1 and CB2) as well as the various ligands and enzymes that interact. The protein KIAA2013 has been found to be expressed within CB2 expressing cells. Both cannabinoid receptors are labeled as class A G-Protein Coupled Receptors, and CB2 is highly expressed within the human spleen and leukocytes. CB2, and by extension KIAA2013, are therefore targets of interest for therapeutic studies looking into diseases such as inflammatory bowel disease and rheumatoid arthritis(PMID:31616248 34752341).

Protocols

Product Specific Protocols
WB protocol for KIAA2013 antibody 27886-1-APDownload protocol
IHC protocol for KIAA2013 antibody 27886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols