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- KD/KO Validated
KIDINS220 Polyclonal antibody
KIDINS220 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21856-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, human brain tissue, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 21856-1-AP is KIDINS220 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KIDINS220 fusion protein Ag16448 相同性解析による交差性が予測される生物種 |
| Full Name | kinase D-interacting substrate, 220kDa |
| Calculated molecular weight | 1672 aa, 187 kDa |
| Observed molecular weight | 186-197 kDa |
| GenBank accession number | BC130610 |
| Gene symbol | KIDINS220 |
| Gene ID (NCBI) | 57498 |
| RRID | AB_10836934 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIDINS220 antibody 21856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. | ||
J Exp Med Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation.
| ||
Sci Adv Kidins220 and Aiolos promote thymic iNKT cell development by reducing TCR signals | ||
Proc Natl Acad Sci U S A A unique PDZ domain and arrestin-like fold interaction reveals mechanistic details of endocytic recycling by SNX27-retromer. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kincaid (Verified Customer) (06-02-2024) | H4 cell lysate run on SDS-PAGE gel. aKIDINS220 diluted in 3% BSA in TBS-tween (0.1%).
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