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- KD/KO Validated
Eg5 Polyclonal antibody
Eg5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 23333-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 15 publications below |
| IHC | See 8 publications below |
| IF | See 6 publications below |
Product Information
23333-1-AP targets Eg5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Eg5 fusion protein Ag18695 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 11 |
| Calculated molecular weight | 1056 aa, 119 kDa |
| Observed molecular weight | 120-130 kDa |
| GenBank accession number | BC136474 |
| Gene symbol | Eg5 |
| Gene ID (NCBI) | 3832 |
| RRID | AB_2716831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Eg5 antibody 23333-1-AP | Download protocol |
| IHC protocol for Eg5 antibody 23333-1-AP | Download protocol |
| IF protocol for Eg5 antibody 23333-1-AP | Download protocol |
| IP protocol for Eg5 antibody 23333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Cancer Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20 | ||
Int J Oncol Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway | ||
Int J Cancer Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma. | ||
Int J Biol Sci KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. | ||
Mol Carcinog Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.
| ||
J Cancer Res Clin Oncol KIF11 serves as a cell cycle mediator in childhood acute lymphoblastic leukemia
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iram (Verified Customer) (01-15-2021) | Eg5 antibody giving a very clean bands and good staining
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