KIF12 Polyclonal antibody

KIF12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Cat no : 12035-1-AP

Synonyms

RP11 56P10.3, Kinesin-like protein KIF12, Kinesin like protein KIF12


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Tested Applications

Positive WB detected inmouse kidney tissue, Y79 cells, mouse pancreas tissue, rat kidney tissue, mouse heart tissue
Positive IHC detected inhuman kidney tissue, human pancreas cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12035-1-AP targets KIF12 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF12 fusion protein Ag2707 相同性解析による交差性が予測される生物種
Full Name kinesin family member 12
Calculated molecular weight 646 aa, 71 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC010626
Gene symbol KIF12
Gene ID (NCBI) 113220
RRIDAB_2280813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KIF12 antibody 12035-1-APDownload protocol
IHC protocol for KIF12 antibody 12035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Am Soc Nephrol

HNF-1beta regulates transcription of the PKD modifier gene Kif12.

Authors - Gong Yimei Y
mouse, humanIF

Kidney Int

Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney.

Authors - Rik Westland
humanWB

Breast Cancer Res Treat

Specific kinesin expression profiles associated with taxane resistance in basal-like breast cancer.

Authors - Tan Min Han MH
humanIHC

J Proteomics

Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.

Authors - Prunotto Marco M
humanWB,IF

J Pediatr

KIF12 Variants and Disturbed Hepatocyte Polarity in Children With a Phenotypic Spectrum of Cholestatic Liver Disease.

Authors - Stalke Amelie