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KD/KO Validated

KIF15 Polyclonal antibody

Name: KIF15 Polyclonal antibody
Brand: Proteintech
SKU: 55407-1-AP

KIF15 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55407-1-AP

Synonyms

KNSL7, KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 55407-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 55407-1-AP
WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells.
WB analysis of Jurkat using 55407-1-AP
WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells.
WB analysis of mouse thymus using 55407-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 55407-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse thymus using 55407-1-AP
IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug.
IHC staining of human testis using 55407-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 55407-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 55407-1-AP
Immunofluorescent analysis of HEK-293 cells using 55407-1-AP (KIF15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 55407-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 55407-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, mouse thymus tissue, HEK-293 cells, MDA-MB-453s cells
Positive IP detected in	mouse thymus tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 10 publications below
WB	See 22 publications below
IHC	See 13 publications below
IF	See 6 publications below

Product Information

55407-1-AP targets KIF15 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	kinesin family member 15
Calculated molecular weight	160 kDa
Observed molecular weight	150-165 kDa
GenBank accession number	NM_020242
Gene symbol	KIF15
Gene ID (NCBI)	56992
RRID	AB_11182836
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

Protocols

Product Specific Protocols
WB protocol for KIF15 antibody 55407-1-AP	Download protocol
IHC protocol for KIF15 antibody 55407-1-AP	Download protocol
IF protocol for KIF15 antibody 55407-1-AP	Download protocol
IP protocol for KIF15 antibody 55407-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Am J Respir Crit Care Med Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis Authors - David Zhang View Article
human	IHC,WB	Cancer Res KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer. Authors - Lin Gao KD Validated View Article
human	IHC	Cancer Cell Int Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer Authors - Enkui Zhang View Article
human	WB,IHC	Cell Physiol Biochem Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma. Authors - Yuan Qiao KD Validated View Article
mouse	WB	Front Cell Dev Biol Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure. Authors - Sai Aung Phyo View Article
human	WB,IHC	Front Oncol KIF15 Promotes Progression of Castration Resistant Prostate Cancer by Activating EGFR Signaling Pathway. Authors - Lin Gao KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIF15 Polyclonal antibody

KIF15 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 55407-1-AP

WB analysis of HEK-293 using 55407-1-AP

WB analysis of Jurkat using 55407-1-AP

WB analysis of mouse thymus using 55407-1-AP

WB analysis of MDA-MB-453s using 55407-1-AP

IP experiment of mouse thymus using 55407-1-AP

IHC staining of human testis using 55407-1-AP

IHC staining of human testis using 55407-1-AP

IF Staining of HEK-293 using 55407-1-AP

FC experiment of HEK-293 using 55407-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis

KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer.

Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer

Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.

Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure.

KIF15 Promotes Progression of Castration Resistant Prostate Cancer by Activating EGFR Signaling Pathway.