Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
19551-1-AP targets KIF19 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 19 |
| Calculated molecular weight | 111 kDa |
| Observed molecular weight | 92 kDa |
| GenBank accession number | NM_153209 |
| Gene Symbol | KIF19 |
| Gene ID (NCBI) | 124602 |
| RRID | AB_10640802 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2TAC6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIF19 belongs to the kinesin-like protein family. The antibody recognizes isoform1 of KIF19 only.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF19 antibody 19551-1-AP | Download protocol |
| IHC protocol for KIF19 antibody 19551-1-AP | Download protocol |
| IF protocol for KIF19 antibody 19551-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Mol Cell Biol Production of transgenic mice by random recombination of targeted genes in female germline stem cells. | ||
J Biol Chem AC6 regulates the microtubule-depolymerizing kinesin KIF19A to control ciliary length in mammals. |