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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF1A Polyclonal antibody (24841-1-AP)

    WB analysis using 24841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24841-1-AP (KIF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KIF1A Polyclonal antibody (24841-1-AP)

    IF Staining of SH-SY5Y using 24841-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KIF1A antibody (24841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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KIF1A Polyclonal antibody

KIF1A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24841-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Kinesin like protein KIF1A, hUnc-104, HUNC104, HUNC 104, C2orf20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24841-1-AP targets KIF1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF1A fusion protein Ag18817 相同性解析による交差性が予測される生物種
Full Name kinesin family member 1A
Calculated molecular weight 1690 aa, 191 kDa
Observed molecular weight191-203 kDa
GenBank accession numberBC111799
Gene Symbol KIF1A
Gene ID (NCBI) 547
RRIDAB_3669455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12756
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KIF1A antibody 24841-1-APDownload protocol
IF protocol for KIF1A antibody 24841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols