Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, A549 cells, HeLa cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
12760-1-AP targets KIF1C in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KIF1C fusion protein Ag3500 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 1C |
| Calculated molecular weight | 1103 aa, 123 kDa |
| Observed molecular weight | 135-140 kDa |
| GenBank accession number | BC034993 |
| Gene Symbol | KIF1C |
| Gene ID (NCBI) | 10749 |
| RRID | AB_2131422 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43896 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF1C antibody 12760-1-AP | Download protocol |
| IHC protocol for KIF1C antibody 12760-1-AP | Download protocol |
| IP protocol for KIF1C antibody 12760-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes | ||