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  • KD/KO Validated

KIF22 Polyclonal antibody

KIF22 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13403-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Kinesin-like protein KIF22, Kinesin-like protein 4, Kinesin-like DNA-binding protein, Kinesin like protein KIF22, Kinesin like protein 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inJurkat cells, human heart tissue, MKN-45 cells, T-47D cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human breast cancer tissue, human brown disease, human cervical cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13403-1-AP targets KIF22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF22 fusion protein Ag4254 相同性解析による交差性が予測される生物種
Full Name kinesin family member 22
Calculated molecular weight 665 aa, 73 kDa
Observed molecular weight 68-73 kDa
GenBank accession numberBC028155
Gene Symbol KIF22
Gene ID (NCBI) 3835
RRIDAB_2249576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).

Protocols

Product Specific Protocols
WB protocol for KIF22 antibody 13403-1-APDownload protocol
IHC protocol for KIF22 antibody 13403-1-APDownload protocol
IP protocol for KIF22 antibody 13403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Rep

PMID: 29229923

Authors - Sara Bizzotto
humanWB

Proteomics

Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.

Authors - Yan Guang-Rong GR
humanWB,IHC

Chin Med J (Engl)

Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways.

Authors - Ze-Yuan Yu
  • KD Validated
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