Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 13403-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13403-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13403-1-AP
IP result of anti-KIF22 (IP:13403-1-AP, 4ug; Detection:13403-1-AP 1:300) with HeLa cells lysate 4000ug.
IHC staining of human brown disease using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded human brown disease slide using 13403-1-AP (KIF22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13403-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

KIF22 Polyclonal antibody

KIF22 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13403-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Kinesin-like protein KIF22, Kinesin-like protein 4, Kinesin-like DNA-binding protein, Kinesin like protein KIF22, Kinesin like protein 4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, human heart tissue, MKN-45 cells, T-47D cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer tissue, human breast cancer tissue, human brown disease, human cervical cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

13403-1-AP targets KIF22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KIF22 fusion protein Ag4254 相同性解析による交差性が予測される生物種
Full Name	kinesin family member 22
Calculated molecular weight	665 aa, 73 kDa
Observed molecular weight	68-73 kDa
GenBank accession number	BC028155
Gene Symbol	KIF22
Gene ID (NCBI)	3835
RRID	AB_2249576
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14807
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).

Protocols

Product Specific Protocols
WB protocol for KIF22 antibody 13403-1-AP	Download protocol
IHC protocol for KIF22 antibody 13403-1-AP	Download protocol
IP protocol for KIF22 antibody 13403-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Sci Rep PMID: 29229923 Authors - Sara Bizzotto View Article
human	WB	Proteomics Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study. Authors - Yan Guang-Rong GR View Article
human	WB,IHC	Chin Med J (Engl) Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways. Authors - Ze-Yuan Yu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13403-1-AP

WB analysis of human heart using 13403-1-AP

IP experiment of HeLa using 13403-1-AP

IHC staining of human brown disease using 13403-1-AP

IHC staining of human cervical cancer using 13403-1-AP